Table 1.
Enumeration and characteristics of CTCs and circulating tumor microembolus (CTMs) identified by NE-iFISH System in PC patients and healthy cases.
| Patients Code | Gender | Age | Location | Histological Type | Stage | Before Treatment | Treatment after 3 Weeks | Outcome (Follow Up for 28 Months) | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CA19-9 (U/ML) | CK+, CD45−, DAPI+ | CK−, CD45−, DAPI+ | CTM | CA19-9 (U/ML) | CK+, CD45−, DAPI+ | CK−, CD45−, DAPI+ | CTM | |||||||||||||||||||||||||||
| Monoploid | Diploid | Triploid | Tetraploid | Multiploid | Sum | Monoploid | Diploid | Triploid | Tetraploid | Multiploid | Sum | Monoploid | Diploid | Triploid | Tetraploid | Multiploid | Sum | Monoploid | Diploid | Triploid | Tetraploid | Multiploid | Sum | |||||||||||
| PC-1 | M | 65 | H | PDAC | IV | 4050 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | 934.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 1 | 3 | 0 | Died |
| PC-2 | M | 51 | BT | PDAC | IV | 13.59 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | 13.59 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | / | 3 | 1 | 0 | 6 | 0 | Died |
| PC-3 | F | 55 | BT | PDAC | IV | 9080 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 1 | 2 | 0 | 10361 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 5 | 5 | 10 | 0 | Died |
| PC-4 | M | 54 | BT | PDAC | IV | 28.12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 0 | 2 | 0 | 727.9 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | / | 10 | 1 | 1 | 25 | 0 | Died |
| PC-5 | M | 39 | H | PDAC | IV | 527.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 1 | 2 | 0 | 423.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 0 | 2 | 0 | Died |
| PC-6 | M | 36 | BT | PDAC | IV | 591.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 1 | 0 | 3 | 0 | 1700 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 14 | 15 | 0 | Died |
| PC-7 | M | 62 | H | PDAC | IV | 964.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 0 | 2 | 0 | 155 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 5 | 0 | 1 | 6 | 0 | Alive |
| PC-8 | M | 59 | H | PDAC | IV | 22.18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 1 | 0 | 4 | 0 | 6.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 1 | 0 | 4 | 1 | Died |
| PC-9 | M | 50 | BT | PDAC | IV | 28068 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 1 | 5 | 9 | 0 | 78,182 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 146 | 146 | 5 | Died |
| PC-10 | F | 70 | BT | PDAC | IV | 3954 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 5 | 0 | 3 | 8 | 2 | 11,356 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 5 | 0 | 7 | 12 | 1 | Died |
| PC-11 | F | 51 | H | PDAC | IV | 132,070 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 9 | 0 | 2 | 11 | 1 | 173,664 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 4 | 0 | 4 | 8 | 1 | Died |
| PC-12 | M | 64 | H | PDAC | IV | 71,019 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 1 | 2 | 0 | 73,780 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 1 | 0 | 4 | 1 | Died |
| PC-13 | M | 59 | H | PDAC | IV | 4925 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 0 | 1 | 0 | Died | ||||||||||||||
| PC-14 | M | 65 | H | PDAC | IV | 2222 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 0 | 1 | 0 | Died | ||||||||||||||
| PC-15 | M | 74 | BT | PDAC | IV | 28.33 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 1 | 0 | 2 | 0 | Lost | ||||||||||||||
| PC-16 | M | 76 | BT | PDAC | IV | 15.13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 0 | 2 | 0 | Died | ||||||||||||||
| PC-17 | M | 50 | H | PDAC | IV | 4423 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 2 | 1 | 3 | 0 | Died | ||||||||||||||
| PC-18 | M | 75 | H | PDAC | IV | 4.25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 0 | 0 | 3 | 0 | Died | ||||||||||||||
| PC-19 | F | 48 | H | PDAC | IV | 10,313 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 0 | 0 | 3 | 0 | Died | ||||||||||||||
| PC-20 | F | 48 | H | PDAC | IV | 442 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 1 | 1 | 3 | 0 | Died | ||||||||||||||
| PC-21 | M | 39 | BT | PDAC | IV | 80,749 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 0 | 0 | 3 | 0 | Died | ||||||||||||||
| PC-22 | M | 76 | BT | PDAC | IV | 19,764 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 4 | 0 | 1 | 5 | 0 | Died | ||||||||||||||
| PC-23 | M | 36 | H | PDAC | IV | 6.09 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 4 | 2 | 8 | 0 | Died | ||||||||||||||
| PC-24 | F | 73 | H | PDAC | IV | 9038 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 6 | 5 | 11 | 22 | 4 | Alive | ||||||||||||||
| PC-25 | F | 64 | H | PDAC | III | 187.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 0 | 1 | 0 | Died | ||||||||||||||
| PC-26 | M | 70 | BT | PDAC | III | 39.40 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 0 | 1 | 0 | Alive | ||||||||||||||
| PC-27 | M | 50 | H | PDAC | III | 12.50 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 1 | 2 | 0 | Alive | ||||||||||||||
| PC-28 | F | 21 | H | PDAC | III | 4.68 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | / | 1 | 1 | 1 | 4 | 0 | Died | ||||||||||||||
| PC-29 | M | 72 | BT | PDAC | III | 848.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 1 | 0 | 4 | 0 | Died | ||||||||||||||
| PC-30 | F | 58 | H | PDAC | II | 2956 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 0 | 2 | 0 | Died | ||||||||||||||
| PC-31 | F | 71 | H | PDAC | II | 18,834 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 1 | 1 | 0 | Died | ||||||||||||||
| PC-32 | M | 56 | BT | PDAC | II | 124.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 0 | 0 | 3 | 0 | Died | ||||||||||||||
| PC-33 | F | 68 | BT | PDAC | II | 124.20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 1 | 2 | 0 | Alive | ||||||||||||||
| PC-34 | F | 58 | H | PDAC | II | 1393 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 1 | 0 | 3 | 0 | Lost | ||||||||||||||
| PC-35 | M | 62 | H | PDAC | II | 942.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 3 | 0 | 4 | 0 | Alive | ||||||||||||||
| PC-36 | M | 71 | H | PDAC | II | 354.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | Died | ||||||||||||||
| PC-37 | M | 65 | BT | PDAC | I | 262.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 3 | 0 | 0 | 3 | 0 | Died | ||||||||||||||
| PC-38 | F | 59 | BT | PDAC | I | 124.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 1 | 0 | 3 | 0 | Lost | ||||||||||||||
| PC-39 | F | 64 | H | PDAC | I | 2088 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | Died | ||||||||||||||
| PC-40 | F | 49 | H | PDAC | I | 90.52 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 1 | 2 | 0 | Died | ||||||||||||||
| BTP-1 | F | PCN | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 2 | 0 | 2 | 0 | |||||||||||||||||||
| BTP-2 | M | AIP | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||
| BTP-3 | M | PCN | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 0 | 1 | 0 | |||||||||||||||||||
| BTP-4 | M | IPMN | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||
| BTP-5 | F | IPMN | 0 | 0 | 0 | 0 | 1 | 1 | 0 | / | 0 | 0 | 1 | 1 | 0 | |||||||||||||||||||
| BTP-6 | F | PCN | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 1 | 2 | 0 | |||||||||||||||||||
| BTP-7 | M | IPMN | 0 | 0 | 0 | 0 | 1 | 1 | 0 | / | 1 | 0 | 0 | 1 | 0 | |||||||||||||||||||
| BTP-8 | M | AC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 1 | 2 | 0 | |||||||||||||||||||
| HC-1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 1 | 2 | 0 | |||||||||||||||||||||
| HC-3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 0 | 1 | 0 | |||||||||||||||||||||
| HC-6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 1 | 1 | 0 | |||||||||||||||||||||
| HC-16 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-17 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 0 | 1 | 0 | |||||||||||||||||||||
| HC-19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 1 | 2 | 0 | |||||||||||||||||||||
| HC-20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 2 | 0 | 0 | 2 | 0 | |||||||||||||||||||||
| HC-21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-22 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 0 | 1 | 0 | |||||||||||||||||||||
| HC-23 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 1 | 1 | 0 | |||||||||||||||||||||
| HC-24 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-26 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-27 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 1 | 0 | 1 | 0 | |||||||||||||||||||||
| HC-28 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 1 | 1 | 0 | |||||||||||||||||||||
| HC-29 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-30 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 0 | 0 | 1 | 0 | |||||||||||||||||||||
| HC-31 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-32 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-33 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
| HC-34 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 1 | 1 | 0 | 2 | 0 | |||||||||||||||||||||
| HC-35 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | / | 0 | 0 | 0 | 0 | 0 | |||||||||||||||||||||
F, Female; M, Male; H, Head; BT, Body and Tail; PC, Pancreatic cancer; BTP, Benign tumors of the pancreas; HC, Healthy control; PDAC, Pancreatic ductal adenocarcinoma; PCN, Pancreatic cystic neoplasms; AIP, Autoimmune pancreatitis; IPMN, pancreatic intraductal papillary mucinous neoplasms; AC, Acute pancreatitis; CTC: circulating tumor cells. CEP-8: chromosome enumeration probes 8; DAPI: 4′,6-diamidino-2-phenylindole; CK18: cytokeratins 18; CD45: cluster of differentiation 45; EpCAM: epithelial cell adhesion molecule; CA 19-9: carbohydrate antigen 19-9. /: Not included in CTC.