Figure 2.

Overexpression of amyC and amyE does not affect significantly α-glucan accumulation. (A) 10⁵ freshly harvested conidia of each strain were inoculated on complete medium and the plates were incubated at 30 °C for 48 h. All constructed strains showed the wild type colony phenotype on solid medium; (B) a large number (5 × 10⁷) of freshly harvested conidia were inoculated in flask with 20 mL complete medium, then the flask was incubated at 30 °C, 150 rpm overnight. Only actA(p)-amyD formed tiny colonies; (C) A large number (2 × 10⁷) of spores of each strain were inoculated in 100 mL complete medium. Samples were grown in flasks at 30 °C with 150 rpm for 24 h. α-glucan was extracted from 1 mg dry cell wall, and then digested to glucose and quantified by an anthrone assay. Results represent the mean of three independent quantification tests with duplicates each time ± standard deviation. The data for each strain were compared with wild type (column 1) individually by Mann Whitney U test. Significant difference (p < 0.05) is indicated by asterisks.