Figure 4.

The absence of AmyD cannot suppress the increased α‑glucan production of agnB- or mutA-overexpression strains. (A) All constructed strains showed the wild-type colony phenotype on solid medium; (B) all strains behaved the same as the wild-type; and (C) α-glucan was extracted from 1 mg of dry cell wall, and then digested to glucose and quantified using an anthrone assay. Results represent the mean of three independent quantification tests with duplicates each time ± standard deviation. The data for each strain were compared with wild type (column 1) individually by a Mann Whitney U test. Significant difference (p < 0.05) was indicated by asterisks.