Figure 3.

Peroxidase and polyphenol oxidase activities during flower development of Brunfelsia calycina and Rosa chinensis. (A) Activities of perioxidases (PODs) in the petals were measured with guaiacol and H₂O₂ as substrates at pH 7.0; (B) Activities of polyphenol oxidases (PPOs) were measured with 4-methylcatechol as substrate at pH 7.0; (C–D) Ten micrograms of total protein extract of different stages of each species was separated by semi-native polyacrylamide gel electrophoresis (PAGE) followed by guaiacol staining in the presence of 0.17 mM H₂O₂ for POD in-gel activity detection, while using 4-methylcatechol for PPO in-gel activity assay (E–F). The details of the values presented in (A,B) are as described in Figure 1, and the details of the lanes presented in (C–F) are as described in Figure 2.