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. 2017 Mar 25;18(4):699. doi: 10.3390/ijms18040699

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Contents of total phenolics and tannins and quantification of tannin constituents by HPLC. (A) Total phenolic contents as tannic acid equivalent (TAE) in the petals of Brunfelsia calycina and Rosa chinensis flowers; (B) Total tannin contents as TAE in the petals; (C) HPLC analysis of the tannin constituent standards; (D) HPLC analysis of the tannin extracts from Rosa chinensis petals at stage 2; (E) HPLC analysis of the tannin extracts from Brunfelsia calycina petals at stage 2; (F) The contents of the identified tannin constituents from HPLC. The statistical details of the values presented in (A,B,F) are as described in Figure 1. GA: gallic acid; GC: Gallocatechin; C: catechin; EGCG: epigallocatechin-3-gallate; EC: epicatechin; GCG: gallocatechin-3-gallate; ECG: epicatechin-3-gallate; CG: catechin gallate.