Figure 3.

Heatmaps of significantly regulated (p < 0.05, 2-way ANOVA) proteins in the brain of zebrafish (Danio rerio). Significant protein responses elicited by: MeHg (A), SeMet (B) and MeHg-SeMet (C) interactions. The number in brackets following the Ingenuity Pathway Analysis (IPA) mapped identifier represent the gel master spot number provided in Figure 2 and Table S1, respectively. Individual proteins found to be regulated in response to (A,B) are labeled with a plus sign (+). Overlapping responses in (A,C) or (B,C) are marked with an asterisk (*) and a hash (#) sign, respectively. Yellow and blue boxes indicate increased and decreased expression levels relative to the treatment groups; the respective log2 fold changes are listed in Table S1.