Figure 5.

Ingenuity Pathway Analysis (IPA) of “upstream regulators” and “diseases and biological functions” of the lists of significantly regulated proteins (p < 0.05, 2-way ANOVA). RICTOR, the rapamycin-insensitive binding partner of the mammalian target of rapamycin (mTOR) was highlighted as a novel master upstream regulator that was predicted to be activated and inhibited in MeHg and SeMet exposed zebrafish brain, respectively. Diseases and biological functions analysis highlighted “cell death” and “necrosis” to be activated by MeHg and inhibited in SeMet exposed zebrafish brain. Activation (displayed in yellow) and inhibition (displayed in blue) of regulators and functions are based on IPA activation z-scores, which combine directional information encoded in the protein expression results with knowledge from the literature to make predictions about likely adverse outcome pathways. Up-regulated proteins are coloured red, down-regulated proteins are coloured green. The full data-sets of the upstream regulator analysis and the diseases and biological functions are presented in Table S2C,D.