Table 1.

Ingenuity Pathway Analysis (IPA) of significantly regulated (p < 0.05, 2-way ANOVA) proteins in the brain of zebrafish (Danio rerio). Statistical significance of the overrepresentation of genes in different “Diseases and biofunctions” is shown as “Score” (−log10 p-value, Fisher’s exact test). Only selected pathways for which in addition to statistical significance (Score > 1.3) activation z-scores were obtained in at least one of the exposure conditions are shown. Activation z-scores combine directional information encoded in the protein expression results with knowledge from the literature to make predictions about the activation or inhibition of likely adverse outcome pathways. The blue and yellow cells indicate whether the displayed pathways were predicted to be inhibited or activated, respectively. The full data-set including the proteins in each pathway is presented in Table S2C.

Diseases and Biofunctions	Score		Activation
	MeHg	SeMet	MeHg	SeMet
Cell death and survival
Cell death	3.0	1.8
Cell death of neuroblastoma cell lines	6.6
Cell death of tumor cell lines	2.8
Necrosis	3.0	1.7
Cell death and survival, neurological disease
Neuronal cell death		2.4
Cellular assembly and organization
Formation of cytoskeleton		2.6
Cellular assembly and organization, cellular function and maintenance
Microtubule dynamics		1.9
Organization of cytoskeleton		2.2
Cellular assembly and organization, tissue development
Formation of filaments		3.5
Cellular compromise
Degeneration of cells		3.9
Cellular growth and proliferation
Proliferation of cells		3.0
Infectious diseases
Viral infection		2.4
Molecular transport
Transport of molecule		2.4
Neurological disease
Degeneration of nervous system		3.1
Neurodegeneration		3.0
Organismal survival
Organismal death		1.9