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. 2017 Apr 1;18(4):732. doi: 10.3390/ijms18040732

Figure 5.

Figure 5

LncRNA-XIST and miR-494 interact with and repress each other. (A) Schematic representation of the predicted target site for miR-494 in lncRNA-XIST. (B) Relative luciferase activities of luciferase reporters bearing wild-type or mutant lncRNA-XIST 48 h following transfection with the indicated miR-494 mimics, mimics negative control (mimics NC), miR-494 inhibitor, or inhibitor NC, in HEK293 cells. ** p < 0.01 vs. mimic NC or inhibitor NC. (C) Relative lncRNA-XIST expression in HEK293 cells transfected with miR-494 mimic, mimic NC, miR-494 inhibitor, or inhibitor NC were analyzed by qRT-PCR. ** p < 0.01 vs. mimic NC or inhibitor NC. (D) Relative miR-494 expression in HEK293 cells transfected with Lv-control, Lv-shRNA, Lv-wt-XIST, or Lv-mt-XIST were analyzed by qRT-PCR. ** p < 0.01 vs. blank group, ## p < 0.01 vs. Lv-wt-XIST. Data are the mean values ± SEM, n = 3. (E) Correlation of expression of lncRNA-XIST and miR-494 in spinal cord tissue collected from SCI model rats. (r = −0.7159, p < 0.001, n = 10). (F) Relative miR-494 expression in spinal cord tissue from SCI model rats injected with Lv-shRNA and Lv-Scramble at 1, 3, 7, and 14 d after SCI. n = 3/group/time point. ** p < 0.01 vs. Lv-Scramble. Data are means ± SEM.