Figure 6.

Delphinidin-induces Ca²⁺ store-dependent calcium oscillations and triggers store-operated Ca²⁺ entry in HT-29 Fura-2 AM loaded cells. (A) Cells in Ca²⁺-free medium were perfused with 5 μM delphinidin. Each colored line represents the Ca²⁺ concentrations of a single cell. The horizontal bar depicts the duration of perfusion. Before the end of the experiment, extracellular Ca²⁺ containing medium was perfused; (B) Mean ± SEM recording from all cells in a representative experiment; (C) Cells in HEPES/Ca²⁺ buffer were pre-treated with 30 μM cyclopiazonic acid (SERCA inhibitor) until the stabilization of Ca²⁺ readouts and then the cells were stimulated with delphinidin 5 μM for 7 min. Each colored line represents a recording from individual cells. The horizontal bar depicts the duration of perfusion; (D) Mean ± SEM data from all cells in a representative experiment. Data are mean ± SEM of 18 cells.