Table 2.
Inositol phosphate accumulation assay to determine agonistic potencies of candidate molecules.
| Compound | EC50 (nM) | pEC50 1 | Emax 1 (%) 2 | n 3 |
|---|---|---|---|---|
| (S)-9 | 0.7 | 9.17 ± 0.19 | 63 ± 6 | ≥3 |
| (R)-9 | 0.6 | 9.26 ± 0.21 | 59 ± 6 | ≥3 |
| (S)-10 | 1.6 | 8.80 ± 0.14 | 64 ± 5 | ≥3 |
| (S)-11 | 2.9 | 8.54 ± 0.15 | 58 ± 4 | ≥3 |
| (S)-13 | 1.4 | 8.87 ± 0.13 | 57 ± 4 | ≥3 |
| (S)-14 | 2.1 | 8.67 ± 0.20 | 42 ± 4 | ≥3 |
| (S)-16 | 1.0 | 8.99 ± 0.20 | 40 ± 4 | ≥3 |
1 Mean values ± SEM; 2 data refer to ghrelin with Emax as 100%; 3 number of independent experiments in duplicates. EC50, Half maximal effective concentration.