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. 2017 Apr 8;18(4):793. doi: 10.3390/ijms18040793

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of propidium iodide (PI) staining in S. aureus. Four S. aureus strains were grown in MHB supplemented with CuO-NP concentrations of 0–5 mM for 3 h and the cells were then subjected to PI (red) staining. The PI fluorescence intensity is given in arbitrary units (a.u.) on the X-axis, and the Y-axis indicates cell counts as measured by flow cytometry. PBS buffer-only and unstained cells were used as controls. PI staining is fluorescent red color. The data are representative of two separate experiments.

Analysis of propidium iodide (PI) staining in S. aureus. Four S. aureus strains were grown in MHB supplemented with CuO-NP concentrations of 0–5 mM for 3 h and the cells were then subjected to PI (red) staining. The PI fluorescence intensity is given in arbitrary units (a.u.) on the X-axis, and the Y-axis indicates cell counts as measured by flow cytometry. PBS buffer-only and unstained cells were used as controls. PI staining is fluorescent red color. The data are representative of two separate experiments.