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. 2017 Apr 12;18(4):808. doi: 10.3390/ijms18040808

Figure 1.

Figure 1

Effect of small ubiquitin-like modifier (SUMO)-specific cysteine protease 1 (SENP1) on the biological characteristics of PC3M prostate cancer cells. (A,B) Lentiviral vector mediated silencing of SENP1 in PC3M cells. PC3M cells were infected with 20 MOI (multiplicity of infection) of PLKO.1-shSENP1 or PLKO.1-shScramble. 48 h after infection, the SENP1 protein expression was detected by Western-blotting (A); At 24 and 48 h after infection, the total RNA was isolated and the mRNA expression of SENP1 was also analyzed by real-time reverse transcript polymerase chain reaction (RT-PCR) (B); (C) PLKO.1-shSENP1 induces apoptosis in PC3M cells. PC3M cells were infected with lentiviral vectors using 20 MOI. Forty-eight hour later, cells were collected, labeled with Annexin-V-APC and cellular apoptosis was analyzed by flow cytometry; (D) Proliferation of PC3M cells transduced with lentiviral vectors. PC3M cells were labeled with dye670, and then infected with lentiviral vectors. At indicated time points after infection, cells were collected and analyzed by flow cytometry. The proliferation index was calculated, using uninfected PC3M cells as control; (E) PLKO.1-shSENP1 inhibits the migration of PC3M cells. Confluent PC3M cells were scratched to generate wounds, at 24 h following infection with lentiviral vectors. 6 and 24 h later, the percentages of wound area filled were determined and analyzed. All the data were obtained from at least three independent experiments, and are shown as mean ± s.e.m. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. PLKO.1-shScramble group; ### p < 0.001 vs. control group.