Figure 3.

Triadimefon exposed embryos exhibit bend spine defects. Representative morphological evaluation and comparison of control (n = 45) and triadimefon treated embryos (31 and 36 embryos examined) (A–C) at 72 hpf, (D–F) 96 hpf and (G–I) 120 hpf. 72 hpf zebrafish embryos exposed to 2 and 4 μg/mL triadimefon show normal development of spine (left panel; green and red arrowheads in B,C vs. blue in A). A still comparable spine morphology is seen for all embryos at 96 hpf (middle panel; blue, green and red arrowheads in D–F). While, no malformations of neural spine were observed in 2 μg/mL triadimefon exposed embryos (green arrowhead in H), the spine of 4 μg/mL triadimefon embryos was severely curved (red arrowhead and dotted region in I, 34/36 embryos examined) at 120 hpf. FON, triadimefon. Embryos are shown to the same scale (bar = 500 µm in I).