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. 2017 Apr 12;18(4):817. doi: 10.3390/ijms18040817

Figure 8.

Figure 8

Cardiovascular defects induced by triadimefon. Representative morphological evaluation of the heart development in embryos (A–C) at 96 hpf and (D–F) 120 hpf. Embryos treated with 2 μg/mL triadimefon presented normal heart development at 96 hpf (green arrowhead in B indicated absence of edema (80/80); green dotted region represents the heart shape in B). Similarly, at 120 hpf heart morphological examination of this group showed normal heart development (E, 89/89). In contrast, at 4 μg/mL triadimefon (C, 80/89) cardiac defects manifested as heart edema (89/89) and a developmental delay of heart tube shape formation (80/89) (red arrowhead and dotted region respectively). A more pronounced phenotype consisting of substantial pericardial edema (red arrowhead, 89/89) and a linear heart tube (89/89) indicating heart malfunction (red dotted region) was the case for 120 hpf 4 μg/mL exposed embryos (F). Arrowhead indicates edema. Dotted blue, green and red lines in A–F map heart shapes. FON, triadimefon. Embryos in A–C are shown to the same scale (bar = 250 µm in C), while embryos in D–F are shown to scale indicated (=500 µm in F).