Figure 10.

Confocal micrographs of the D. rerio gill apparatus. Sections labeled with a mouse monoclonal antibody against metallothionein (MT) (green–Fluorescein Isothiocyanate (FITC) labeled); nuclei labeled with propidium iodide (red); (A) no MTs expression in the gills of the control group; (B) after 96 h of exposure to 7.7 µg/L of HgCl₂, MTs immunoreactivity strongly appear in both the primary and secondary epithelium; (C) after 96 h of exposure to 38.5 µg/L of HgCl₂, the intensity of staining lightly decrease compared to the basal condition in both the filament and lamellar epithelium. All bars 75 µm.