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. 2017 Apr 17;18(4):844. doi: 10.3390/ijms18040844

Figure 4.

Figure 4

Interaction of PPAR-γ with TLR4 in HMEC-1 cells in response to LPS. (A) Western blotting of TLR4 in HMEC-1 cells. The HMEC-1 cells were pre-incubated with pioglitazone (20 μM) for 6 h followed by LPS (500 ng/mL) stimulation for 48 h; (B) Relative density of TLR4 protein bands determined by densitometry of the blots, (C) TLR4 mRNA levels; (D) Western blotting of PPAR-γ in HMEC-1 cells. The HMEC-1 cells were transfected with 100 nM control siRNA or TLR4 siRNA for 24 h followed by LPS (500 ng/mL) stimulation for 48 h; (E) Relative density of PPAR-γ protein bands determined by densitometry of the blots; (F) PPAR-γ mRNA levels. Values are mean ± S.D. * p < 0.05 vs. control or Veh, † p < 0.05 vs. Con(control) siRNA, ‡ p < 0.05 vs. control + LPS or Veh + LPS. n = 4 for each group. LPS, lipopolysaccharide; TLR4, toll-like receptor 4, PPAR-γ, peroxisome proliferator-activated receptor γ.