Table 4.
The primer pairs used in the semi-quantitative real-time RT-PCR analysis of the selected genes.
| Gene Symbol 1 | GenBank 2 | Sequence (5′–3′) 3 | Amplicon Size (bp) |
|---|---|---|---|
| PHGDH | NM_001123162 | F: GCGGTTTGGTTTAGGTGTTTC | 113 |
| R: AAGGGTCCAGGCTATCACT | |||
| PSPH | NM_001243221 | F: CTGCAGGCTCCAGTTTAGTT | 97 |
| R: CTCGCAGAGTCTTTACCAACA | |||
| SCD | NM_213781 | F: CCCAAGGCAGACAAGAGAATAG | 91 |
| R: GTGTTGACGACTGAGGTTACAG | |||
| CIDEC | NM_001112689 | F: CCAACTCTCCCTCTCCCATAA | 106 |
| R: CATGTTCAGGCAACCAATGAAG | |||
| AMD1 | XM_003121345 | F: TCCACAAGTCAAGTCCTCTAATG | 108 |
| R: CCATGGAGAGGAACGAATCAA | |||
| ZIC1 | XM_003358599 | F: CGACCGACGCTTTGCTAATA | 97 |
| R: GTAGGACTTGTCGCACATCTT | |||
| ERLEC1 | XM_003125147 | F: GCTGGCTATCCTTTGTACTCTC | 109 |
| R: CAACACTGCTTGTGGACATTT | |||
| DNAJA1 | NM_001244163 | F: GGTGGTAAGAAAGGAGCAGTAG | 93 |
| R: CTGAACCATTCCAGGTCCTATT | |||
| ZNF181 | NM_001244818 | F: GCCTTCAGCCAAAGCAAATC | 85 |
| R: AGGCTTTCCCACATTCACTAC | |||
| HPRT1 4 | NM_001032376 | F: GCTATGCCCTTGACTACAATGA | 102 |
| R: TTGAACTCTCCTCTTAGGCTTTG |
1 PHGDH = Phosphoglycerate dehydrogenase; PSPH = Phosphoserine phosphatase; SCD = Stearoyl-CoA desaturase; CIDEC = Cell death-inducing DFFA-like effector c; AMD1 = Adenosylmethionine decarboxylase 1; ZIC1 = Zic family member 1; ERLEC1 = Endoplasmic reticulum lectin 1; DNAJA1 = DnaJ (Hsp40) homolog, subfamily A, member 1; ZNF181 = Zinc finger protein 181; HPRT1 = Hypoxanthine phosphoribosyltransferase 1; 2 The accession number of the cDNA sequences retrieved from the GenBank database (http://www.ncbi.nlm.nih.gov) for primer design; 3 F = a forward primer, and R = a reverse primer; and 4 HPRT1 was selected as an internal control reference gene for the purpose of normalization of the expression of other gene.