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. Author manuscript; available in PMC: 2017 May 2.
Published in final edited form as: J Neurooncol. 2016 Mar 9;128(1):93–100. doi: 10.1007/s11060-016-2081-5

Fig. 1.

Fig. 1

Study design. Samples were collected from a patient undergoing sequential lumbar punctures for the diagnosis and treatment of melanoma leptomeningeal metastases (1). Samples were centrifuged to separate plasma from blood cells and CSF from tumor cells (2). After DNA extraction (3) cellular DNA from CSF and blood underwent exome sequencing to identify tumor mutations. cfDNA from plasma and CSF was utilized to determine the mutant fraction of the known BRAF mutation via ddPCR