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. 2017 May 1;149(5):595–609. doi: 10.1085/jgp.201611699

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© 2017 Çetiner et al.

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Figure 5. — The puse-step-pulse protocol reveals adaptable fractions of channel populations in PA and EC. (A–D) The low- and high-threshold subpopulations of native channels in PA (A and B) and EC (C and D) exhibit distinctive adaptive behaviors. Exposure of excised patches to prolonged moderate tension produces massive inactivation of the native low-threshold channel population. (A) The first 0.1-s pressure pulse invokes ∼50% of total patch conductance, engaging the low-threshold population. The following 10-s step of variable amplitude conditions the low-threshold population, and the last pulse, equal in amplitude to the first, reveals the much smaller population that remains active after the conditioning step. When the same protocol utilizes saturating pulses (engaging the entire channel population) and a broader range of conditioning steps, a larger fraction of the population remains active. The experiment shows that the low-threshold population in PA is especially prone to inactivation. Red arrows indicate current levels produced by the remaining channel population at the end compared with the amplitude of response to the initial pulse (black arrows).