Figure 3.

Cell death caused by centrosome/SAC loss reduces the neural progenitor pool. (A) Diagram of the third instar brain indicating optic lobes and central brain, as well as the locations of medullary and central brain NBs. (B and C) Double mutant brains possessed significantly fewer NBs per hemisphere (B) or when corrected for smaller brain size (C). (D) Casp3 (green) and the NB marker Dpn (red) indicate that some apoptotic cells were NBs. The arrows in D–D′′ highlight a cell that stained positive for both Casp3 and Dpn. D′ shows the Casp channel only, revealing dying cells; D′′ shows the Dpn channel only, marking NBs. (E) p35 (green) was not expressed in WT brains. Ecad outlines brain morphology. (F) Misexpressing p35 by 1407-Gal4 drove robust p35 expression, particularly in NBs. E and F are maximum-intensity projections. (G) Expressing p35 in mad2 sas-4 increased brain size. Error bars represent means ± SD.