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. 2017 May 1;216(5):1387–1404. doi: 10.1083/jcb.201608038

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© 2017 Simões et al.

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Figure 2. — Sna family proteins and DEcad transcriptional down-regulation are not essential for NB ingression. (A–C) Embryos expressing Sna::GFP (green) and stained for Ac (red) and DEcad (blue). I, intermediate row; L, lateral row; M, medial row. Medial proneural clusters express Ac at early stage 7 (A, arrowheads), preceding lateral expression seen at the onset of stage 8 (B); Sna is robustly expressed in ingressed NBs during stage 9 (C). (D and E) Sna (green) is expressed by VM cells but not in Ac-positive medial proneural clusters (arrowheads) at stage 7 (D) and early stage 8 (E). (F) Ingressing NBs (asterisks) express Sna and enrich Ac at mid–stage 8. (G) Two medial NBs flanking the midline expressing Ac and Sna at stage 9. Bars: (A–C) 50 µm; (D and E) 10 µm; (F and G) 5 µm. (H) Mean apical area loss during NB ingression in mock-injected controls (blue) and embryos depleted for Sna, Esg, and Wor (red) by RNAi. (I) Mean apical area loss during NB ingression in Spider::GFP controls (blue) and shg^R69 mutants expressing ubi-DEcad::GFP (red). Data presented are means ± SEM.