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. 2017 May 1;216(5):1387–1404. doi: 10.1083/jcb.201608038

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© 2017 Simões et al.

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Figure 8. — Canoe is required for NB ingression. (A) Stills from time-lapse videos of ingressing NBs in water-injected Sqh::GFP GAP43:mCherry embryos (control) and canoe-RNAi embryos. Arrowheads indicate the detachment of junctional myosin from an NB edge during ingression. Bars, 5 µm. (B) Apical area loss during ingression in control (22 NBs, four embryos) and canoe-RNAi embryos (24 NBs, three embryos). Data presented are means ± SEM. (C) Fractions of time ingressing NBs spend contracting and expanding in control and canoe-RNAi. *, P = 0.037 (KS test). Data presented are means ± SD. (D) Amplitude and duration of individual contractions/expansions during ingression in control and canoe-RNAi. Median amplitudes for control/RNAi (bars; squared micrometers/minute): contractions, 3.38/2.12; ***, P = 3 × 10⁻⁴; expansions, 1.15/1.08; ns (not significant), P = 0.599. Median duration for control/RNAi (seconds): contractions, 61.57/50.71, *, P = 0.012; expansions, 17.17/19.04, ns, P = 0.385 (KS test); n = 154–297 events per condition. (E) Amplitude and duration of apical contractions and expansions in NICs in control and canoe-RNAi embryos. Median amplitudes for control/RNAi (bars; squared micrometers/minute): contractions, 3.09/2.38; **, P = 0.005; expansions, 3.94/2.44; ****, P = 7.2 × 10⁻⁷. Median duration for control/RNAi (seconds): contractions, 33.35/33.73; ns, P = 0.983; expansions, 38.12/35.45; ns, P = 0.3613 (KS test). n = 275–401 events per condition.