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. 2017 May 1;216(5):1489–1499. doi: 10.1083/jcb.201608081

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© 2017 Salinas et al.

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Figure 1. — Ultrastructural analysis of subretinal vesicles in rds^−/− mice. (A) EM images of retinal cross sections from rds^−/− (left) and WT (right) mice at P21. (B) Gaussian histogram of Feret diameters for extracellular vesicles in rds^−/− retinas (n = 250 pooled from two animals). Means ± SD are shown. (C) An example of extracellular vesicle budding from the ciliary membrane of an rds^−/− rod. (D) Immunogold labeling of rhodopsin (4D2 mAb) in the distal cilium and extracellular vesicles of a rod photoreceptor in rds^−/− and WT retina. Insets show magnified areas marked by red and blue rectangles. Bars: (A and D, main images) 500 nm; (C) 200 nm; (D, insets) 100 nm.