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. 2017 May 1;216(5):1489–1499. doi: 10.1083/jcb.201608081

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© 2017 Salinas et al.

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Figure 4. — Ectosome occurrence in 10-d-old WT and rds^+/− mice. (A) EM image of a WT rod with ectosomes next to the distal ciliary end. (B) Immunogold labeling of rhodopsin (4D2 mAb) in these ectosomes. (C) EM image of an rds^+/− retina with three ectosome clusters next to developing cilia. Red arrows point to ectosomes. Bars: (A and B) 200 nm; (C) 500 nm. (D) The prevalence of cilia with adjacent ectosomes in WT and rds^+/− retinas. Between 350 and 450 cilia were analyzed in three retinas of each genotype. Data are averaged among three retinas and shown as SEM.