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. 2017 May 1;216(5):1321–1335. doi: 10.1083/jcb.201609071

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© 2017 Winsor et al.

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Figure 3. — Mutant variants show defects in GMPPNP-induced crossover. (A) Normalized PIFE (n = 3 replicates, ±SEM) over time when each of the indicated Cy3-cytoDATL variants is mixed with GMPPNP. (B) Zoomed-in view of the first 600 s of the trace in A. Final concentrations after mixing were 1 µM Cy3-cytoDATL and 1 mM nucleotide. All traces were repeated with independent protein preparations with similar results. WT, wild type.