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. 2017 May 1;216(5):1439–1453. doi: 10.1083/jcb.201610063

Figure 7.

Figure 7.

Tj modulates E-cadherin expression for somatic cells to intermingle with PGCs and adopt IC cell fate. (A–F) Late-L3 mock control (ctrl; A, C, and E), tj-knockdown (B), tj-overexpressing (D), and shg-knockdown (F) mosaic gonads with GFP (green, flip-out clones), E-cad (gray), Tj in A–D (red in A and B, blue in C and D, ICs), and Vasa in E and F (blue, PGCs). tjRNAi and shgRNAi flip-out cells do not intermingle with PGCs and only locate in apical and basal regions of mosaic gonads. tj-overexpressing flip-out clones exhibit low expression of E-cadherin. Dashed lines mark forming TFs. Bars: (A and C) 10 µm; (E) 20 µm.