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. 2017 Mar 24;7:407. doi: 10.1038/s41598-017-00294-6

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Porcine recellularized arteries after 5 days in culture. Representative scanning electron microscopy (SEM) images of the luminal side of the arteries: (A) decellularized artery with no cells, (B and C) non polymer-coated recellularized arteries, white arrows are pointing out gaps between cells, (D) polymer-coated decellularized arteries, (E and F) polymer-coated recellularized arteries showing the formation of a continuous cell monolayer. (Magnification; (A,C,D and F) x4000, (B and E) x1600). (G and H) Transmission electron microscopy (TEM) analyses of sections cut in perpendicular to the long axis of the artery of (G) native arteries and (H) polymer-coated recellularized arteries. (H) White asterisk show cell adhesion between two cells and white arrows point out adhesion between the cell and the luminal side of the vessel. L: lumen, EC: endothelial cells, N: nucleus, ca: caveolae, er: rough endoplasmic reticulum, m: mitochondria, scale bar indicates: (A) 10 μm, (B) 5 μm.