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. Author manuscript; available in PMC: 2017 May 2.
Published in final edited form as: Microvasc Res. 2012 May 25;84(2):161–168. doi: 10.1016/j.mvr.2012.05.003

Figure 4. ZO-1 Protein Expression Changes in AGP-treated RBMECs.

Figure 4

ZO-1 protein expression was determined on cell lysates collected from RBMECs treated with α-1-acid glycoprotein (AGP) for 6, 12, 24, 48 and 72 hrs, using Western blot analysis. AGP treatment at 50 μg/ml (horizontal bars) and 500 μg/ml (diagonal line) were compared to controls (open bars). ZO-1 protein expression was normalized by β-actin expression with representative blots shown above the bar graph. Statistical analysis used One-way ANOVA with Student Neuman Keuls post-hoc. * indicates p-value <0.05; ** indicates p-value <0.01; *** indicates p-value <0.001 compared to respective control; n = 3–7. Two-way ANOVA results indicate that ZO-1 protein levels are significantly different following 50 μg/ml AGP treatment versus 500μg/ml treatment at 6 and 12 hrs.