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. Author manuscript; available in PMC: 2017 May 2.
Published in final edited form as: Microvasc Res. 2012 May 25;84(2):161–168. doi: 10.1016/j.mvr.2012.05.003

Figure 5. AGP Effects on AP-1 Family DNA Binding Activity in RBMECs.

Figure 5

c-Fos and c-Jun DNA binding activity was examined using nuclear extract collected from RBMECs treated with α-1-acid glycoprotein (AGP); 50 μg/ml (A) or 500μg/ml (B) for 1, 3, 6, 12, 24, 48, and 72 hrs (See materials and methods). AGP stimulates/inhibits nuclear c-Fos (left side) and c-Jun (right side) activity toward their consensus sequence (TRE) in a time- and concentration-dependent manner. Both 50 μg/ml (A) and 500μg/ml (B) AGP increased c-Fos activity at 1 hr while inhibition of c-Fos was seen at 3 and 12 hrs. An increase of c-Jun activity was observed following high-concentration AGP treatment at 1 hr while the low-concentration AGP did not change c-Jun activity during the treatment period tested. * indicates p-value <0.05; ** indicates p-value <0.01; *** indicates p-value <0.001, compared with respective control; n = 2–4.