Table 1.

Primer sets for Real-time PCR

Target	Forward (5′ to 3′)	Reverse (5′ to 3′)	Size (bp)	Reference
Cytokines
IL-10	GCCAAGCCTTGTCAGAAATGA	TTTCTGGGCCATGGTTCTCT	75	(Gayle et al., 2004); NM_012854
TNF-α	TGGCCAATGGCCATGGATCTCAA	ATGAAGTGGCAAATCGGCTGAC	147	IDT; NM_012675.2
IL-1β	TGTCCTGTGTGATGAAAGACGG	TTGGGTATTGTTTGGGATCCA	69	IDT; NM_031512.2
IL-6	AGCCACTGCCTTCCCTACTT	GCCATTGCACAACTCTTTTCTC	154	(Lin et al., 2009); NM_012589.1
TJ Proteins
Occludin	GTTTACTGGCAGAACTCGAC	CCAGCATCTGTCTAGGTTTTC	247	IDT; NM031329.2
ZO-1	GGCTGTCTCAACTCCTGTAA	CGCCAGCTACAAATATTCCG	299	IDT; NM_001106266.1
Claudin-2	TATGTTGGTGCCAGCATTGT	ACTCCACCCACTACAGCCAC	266	(Abuazza et al., 2006); NM_001106846.2
Claudin-5	CGCTTGTGGCACTCTTTGT	ACTCCCGGACTACGATGTTG	168	(Wongdee et al., 2010); NM_031701.2
JAM-1	ACCCCGTGACAGCCTTTGATA	TCAGCTCCACATCATCCATGT	105	IDT; NM_053796.1
GAPDH	TGACTCTACCCACGGCAAGTTCAA	ACGACATACTCAGCACCAGCATCA	141	IDT; NM_017008.3

IDT: Integrated DNA Technologies.