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. 2017 Mar 23;18(5):745–764. doi: 10.15252/embr.201643191

Figure EV1. NELF‐E depletion alleviates A20 expression at DSB sites.

Figure EV1

  1. A flowchart depicting the layout of an experiment designed to determine the effect of NELF‐E on the transcription of A20 gene following DSB induction.
  2. Western blot shows NELF‐E knockdown in HeLa cells at 72 h after siRNA transfection. β‐actin was used as a loading control.
  3. Graph displays the effect of NELF‐E depletion on the transcription activity of A20 gene upon Cas9‐induced DSBs. Results show that NELF‐E negatively regulates A20 expression at DSB sites. Data represent the mean of two biological repeats.