Table 3.
Antibodies used in this study
| Name | Source | Dilution for Western blot | Dilution for IF |
|---|---|---|---|
| Primary antibodies | |||
| Anti‐NELF‐E | Abcam ab170104 | 1:3,000 | 1:250 |
| Anti‐GFP | Abcam ab290 | 1:1,000 | |
| Anti‐NELF‐A/WHSC1 | Abcam ab85852 | 1:2,000 | |
| Anti‐NELF‐B/COBRA1 | Abcam ab167401 | 1:2,000 | |
| Anti‐β‐actin | Sigma A5441 | 1:15,000 | |
| Anti‐PARP1 | Enzo ALX‐210–895‐R100 | 1:3,000 | |
| Anti‐Flag | Sigma F1804 | 1:1,000 | |
| Anti‐H3 | Abcam ab1791 | 1:2,000–20,000 | |
| Anti‐γH2AX (ser139) | Millipore 05‐636 | 1:2,500 | |
| Anti‐γH2AX | Cell Signaling 2577 | 1:1,000 | |
| Anti‐RNA polymerase II (POLR2A/RPB1) | Santa Cruz sc‐899 | 1:1,000 | |
| Anti‐poly‐ADP‐ribose binding reagent | Millipore MABE1031 | 1:4,000 | 1:400 |
| Anti‐Rad51 | GeneTex GTX118249 | 1:1,500 | |
| Anti‐Ku80 | Santa Cruz sc‐1484 | 1:200 | |
| Secondary antibodies | |||
| Anti‐mouse (IgG)‐HRP | Amersham | 1:10,000 | |
| Anti‐rabbit (IgG)‐HRP | Jackson ImmunoResearch 111–035‐003 | 1:20,000 | |
| Anti‐goat (IgG)‐HRP | Abcam ab6885 | 1:10,000 | |
| Donkey anti‐rabbit Alexa Fluor 488 | Invitrogen A21206 | 1:500 | |
| Goat anti‐mouse Alexa Fluor 680 | Invitrogen A21057 | 1:500 | |
| Donkey anti‐mouse Alexa Fluor 568 | Invitrogen A10037 | 1:500 | |