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Photomicrograph of sperms, A. Sperm with normal DS-DNA, B. Sperm with PSS-DNA, C. Sperm with SS-DNA, D. Dead sperm with stained cytoplasm, E. Live sperms with colorless cytoplasm, F. Sperm with immature ChC, and G. Sperm with ChC. A, B, C. AO staining, D, E. Eosin-nigrosin staining, F and G. Aniline blue staining.
AO; Acridine orange, DS-DNA; Double strand DNA, PSS-DNA; Partly single stranded DNA, SS-DNA; Single strand DNA, and ChC; Chromatin condensation.
