Fig.3.

Cross section from seminiferous tubules, A. Control, B. ATZ alone, C. ATZ+VitE, D. ATZ+T, E. ATZ+VitE+T. A1. Representing intact spermatogenesis (IS) in control group with no apoptosis, A2, B1. Note germinal cells dissociation (GCD), edema (E), damaged spermiogenesis (DS) and decreased Sertoli cells (SC) in ATZ-received group, representing intensive apoptosis in B2, C1. See germinal cells integrity (GCI) and ameliorated spermiogenesis in ATZ+VitE-received group with decreased apoptosis at germinal cells level, C2, D1. Note normal Sertoli cells distribution (SC) and ameliorated spermiogenesis (AS) in ATZ+T-received group, representing diminished apoptosis D2, E1. Represents normal Sertoli cells distribution, ameliorated spermatogenesis and spermiogenesis ratio associated with diminished apoptosis E2. H&E and TUNEL Stainings. F. Effects of VitE- and T-alone and their simultaneous administration on ATZ-reduced number of Sertoli cells per one seminiferous tubule. All data are presented in mean ± SD.

^a vs. ^b; Significant at P<0.01, c vs. b; Significant at P<0.01 and d vs. b and c; Significant at P<0.05, ATZ; Atrazine, VitE; Vitamin E, T; Testosterone and TUNEL; Terminal deoxynucleotidyl transferase (dUTP) nick-end-labeling.