Figure EV4. TNBS‐induced colitis in cell‐specific DP1‐deficient mice.

1. Body weight loss, disease activity index (DAI), and colon length of DP1^F/F/Tie2^Cre mice, DP1^F/F/Villin^Cre mice, DP1^F/F/LysM^Cre mice, and DP1^F/F/SM22^Cre mice in response to TNBS treatment.
2. Quantitative measurement of vascular permeability by dye leakage in the colonic mucosa from TNBS‐challenged DP1^F/F/Tie2^Cre and DP1^F/F mice. The mice were sacrificed at day 6.
3. TUNEL assay (left) and quantitation (right) in colonic tissues from TNBS‐challenged DP1^F/F/Villin^Cre and DP1^F/F mice. The arrows indicate the TUNEL⁺ cells (left). Scale bar: 100 μm. DP1^F/F, n = 7; DP1^F/F/Villin^Cre, n = 8.
4. Representative immunofluorescent staining (left) and quantitation (right) of CD301⁺CD68⁺ cells in colonic tissues from TNBS‐challenged DP1^F/F/LysM^Cre and DP1^F/F mice. The arrows indicate the CD301⁺CD68⁺ cells (left). Scale bar: 100 μm. DP1^F/F, n = 8; DP1^F/F/LysM^Cre, n = 6.

Data information: Data are shown as mean ± SEM. Statistical significance was determined using two‐way ANOVA followed by a Bonferroni post hoc test (A) and unpaired Student's t‐tests (B–D). (A, B) *P < 0.05, **P < 0.01 compared with DP1^F/F. DP1^F/F, DP1^F/F/Tie2^Cre, DP1^F/F/LysM^Cre, DP1^F/F/SM22^Cre n = 6; DP1^F/F/Villin^Cre, n = 7. (C, D) **P < 0.01 vs. DP1^F/F.