Figure EV2. ∆F4L∆J2R VACV retains much of the replication proficiency of wild‐type VACV in bladder cancer cells.

Growth curves for the indicated VACV mutants or WT VACV. Subconfluent cells were infected at a multiplicity of infection of 0.03 PFU/cell. Cultures were harvested at the indicated times and titered on BSC‐40 cells.

1. Normal human kidney epithelial cells grown under normal serum conditions (left) and 0.1% FBS (right).
2. Panel of human bladder cancer cell lines (exception: MB49‐luc, murine urothelial carcinoma) cultured in vitro with 10% FBS.

Data information: Mean ± SEM is shown and data represent at least two independent lysates titered in duplicate.