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. 2017 Apr 5;18(5):797–808. doi: 10.15252/embr.201643270

Figure 3. USP26 stabilization of SMAD7 enhances TGF‐β receptor degradation.

Figure 3

  1. 293T cells transfected with FL‐SMAD7 (1 μg), GFP‐USP26 (4 μg), GFP‐USP26 C/S (4 μg) or control vector. Whole‐cell extracts were probed with the indicated antibodies. * denotes background band.
  2. 293T cells stably expressing lentiviral knockdown vectors targeting USP26 or control vector were transfected with FL‐SMAD7 (1 μg). Whole‐cell extracts were probed with the indicated antibodies. * denotes background band.
  3. 293T cells stably expressing lentiviral knockdown vectors targeting USP26 or control vector were lysed, and whole‐cell extracts were probed with the indicated antibodies.
  4. 293T cells stably expressing lentiviral knockdown vectors targeting USP26 or control vector were transfected with FL‐SMAD7 (1 μg); the cells were treated with MG132 (5 μg/ml) as indicated overnight and subsequently lysed. Whole‐cell extracts were probed with the indicated antibodies.
  5. 293T cells stably expressing a USP26 knockdown vector or control vector were treated with MG132 (5 μg/ml) as indicated overnight and subsequently lysed. Whole‐cell extracts were probed with the indicated antibodies.
  6. 293T cells transfected with TβRI (1 μg), GFP‐USP26 (5 μg), GFP‐USP26 C/S (5 μg), or control vector. Whole‐cell extracts were probed with the indicated antibodies.
  7. 293T cells were transfected with TβRI (5 μg) and either control or knockdown vectors targeting USP26. Whole‐cell extracts were probed with the indicated antibodies.
  8. 293T cells stably expressing lentiviral knockdown vectors targeting USP26 or control vector were lysed, and whole‐cell extracts were probed with the indicated antibodies.
  9. Immunoblots of biotinylated TβRI in MDA‐MB‐231 stably expressing lentiviral knockdown vectors targeting USP26 or control vector.
  10. 293T cells transfected with FL‐TRICA and knockdown vectors targeting USP26, and HA‐tagged ubiquitin. Following immunoprecipitation of FL‐TRICA, lysates were resolved by SDS–PAGE and probed with the indicated antibodies.

Source data are available online for this figure.