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Immunohistochemistry protocols for USP26 and pSMAD2
| Antibody | Manufacturer | Autostainer | Dilution | Antigen retrieval | Block (min) | Antibody incubation | Detection kit used |
|---|---|---|---|---|---|---|---|
| USP26 (ab101650) | Abcam | BOND‐MAX | 1/50 | pH 9, 20 min | None | 15 min | Bond Polymer Refine Red Detection Kit |
| pSMAD2 (3108) | Cell Signaling | BOND‐MAX | 1/100 | pH 9, 20 min | None | 15 min | Bond Polymer Refine Red Detection Kit |
Immunohistochemistry protocols for USP26 and pSMAD2 detection in glioblastoma samples.
