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. 2017 Apr 4;9(5):589–604. doi: 10.15252/emmm.201607027

Figure 4. IL‐23‐induced psoriasis‐like skin disease is attenuated in RIG‐I−/− mice.

Figure 4

  1. Representative photographs of the ears of WT mice (upper panel) and RIG‐I−/− mice (lower panel) after intradermal injection with PBS or IL‐23 (500 ng) on every other day for eight times, n = 4–5 per group.
  2. The ear thickness of WT and RIG‐I−/− mice on the indicated day presented relative to day 0. Significant differences are indicated: P = 0.0033, one‐way ANOVA, n = 4–5 per group (mean ± SEM).
  3. Representative H&E staining of the ears treated as in (A), n = 4–5 per group. Scale bar: 50 μm.
  4. Acanthosis of WT and RIG‐I−/− mice treated with PBS or IL‐23. Significant differences are indicated: two‐tailed Student's t‐test, n = 3–8 per group (mean ± SEM).
  5. Representative immunostaining of Ki67 in ear skin derived from WT and RIG‐I−/− mice treated with PBS or IL‐23, n = 3–6 per group. Scale bar: 20 μm.
  6. Quantitation of Ki67+ cells in ear skin derived from WT and RIG‐I−/− mice treated with PBS or IL‐23. Significant differences are indicated: two‐tailed Student's t‐test, n = 3–6 per group (mean ± SEM).
  7. Western blot analysis of RIG‐I and p‐IκBα expression after treatment as in (A).
  8. ELISA detection of IL‐23p19 protein levels in supernatants of ear skin homogenates derived from indicated groups. Significant differences are indicated: two‐tailed Student's t‐test, n = 3 per group (mean ± SEM).

Source data are available online for this figure.