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. 2017 Mar 10;9(5):605–621. doi: 10.15252/emmm.201607204

Figure EV2. TFE3 regulates β‐oxidation during starvation.

Figure EV2

  • A–D
    Haematoxylin and eosin (H&E) staining (A), Oil Red O (B) and electron microscopy images (C) with the relative quantification of the lipid droplets and liver triglyceride (TG) levels (D) of livers isolated from fed and 24‐h‐fasted Tfe3 KO and control mice (n = 5 per group) (scale bars H&E: 20 μm; scale bars Oil Red O: 50 μm). Data are presented as mean ± SEM. Student's two‐tailed t‐test: ****P < 0.0001; **P = 0.0041; *P = 0.0288.
  • E
    Quantification of mRNA levels of genes involved in lipid metabolism in livers from WT and Tfe3 KO mice treated as indicated (n = 3 per group). Data are presented as mean ± SEM. Student's two‐tailed t‐test: Tfe3 ***P < 0.001; Cd36 *P = 0.0162; Cyp7a1 *P = 0.05; Fgf21 **P = 0.0011; Cpt1α *P = 0.0307; Pgc1α *P = 0.0147; ApoA4 *P = 0.0147; Cyp17a1 ***P = 0.0005; Cyp4a10 **P = 0.0056; Cyp4a14 **P = 0.0048; Fasn **P = 0.0275; Srebp1c *P = 0.0255.
  • F
    Quantification of mRNA levels of genes involved in lipid metabolism in primary hepatocytes from WT and Tfe3 KO mice treated as indicated. Data are presented as mean ± SEM. Student's two‐tailed t‐test: Tfe3 **P < 0.003; Acot1 ***P = 0.0007; Pgc1α *P = 0.0159; Cd36 ***P = 0.0002.
  • G
    Expression of genes involved in lipid metabolism in livers from HDAd‐PEPCK‐TFE3 injected mice (n = 3 per group). Values were normalized to control livers (dashed line). Data are presented as mean ± SEM. Student's two‐tailed t‐test: Cd36 **P = 0.0018; Cpt1α *P = 0.0449; Pgc1α *P = 0.0207; ApoA4 ***P = 0.0004; Cyp17a1 *P = 0.0162; Cyp4a10 **P = 0.0067; Cyp4a14 *P = 0.0480.