Table 3.
Viability of cells stimulated for 4 and 24 h with different biofilms, compared with the medium‐only control. Values were obtained using multiple comparisions by ANOVA with post‐hoc t‐tests
| Study time point and method used to determine cell viability | mix live | pg bf live | sm bf live | mix dead | pg bf dead | sm bf dead |
|---|---|---|---|---|---|---|
| 4 h | ||||||
| AlamarBlue® | 0.086 | 1.000 | 1.000 | 0.001 | 0.000 | 0.018 |
| Histone | 0.0025 * | 0.008 | 0.003 | 0.006 | 0.0016 * | 0.004 |
| 24 h | ||||||
| AlamarBlue® | 0.004 | 0.0025 * | 0.003 | 0.0015 * | 0.0004 * | 0.011 |
| Histone | 0.0004 * | 0.019 | 0.0013 * | 0.017 | 0.008 | 0.023 |
bf, biofilm; dead, fixed; mix, mixed species; pg, Porphyromonas gingivalis; sm, Streptococcus mitis.
Bold denotes statistically significant differences compared with medium control.
*Significant after Bonferroni correction.