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. 2017 Mar 20;36(9):1215–1226. doi: 10.15252/embj.201695861

Figure 2. miR‐31a is required in adult glial progenitor cells to prevent glial apoptosis.

Figure 2

  • A–D
    Number of anti‐repo‐positive glia at 7 days of age. Glia counts in the experimental samples are represented as a percentage of the average number of glia in central brains of the Gal4/+ controls for each panel. Data were analysed using one‐way ANOVA with post hoc Tukey analysis. Error bars represent SEM. For each Gal4 driver tested, Gal4/+ was compared with the UAS‐miR‐31a sponge transgene/+ and with Gal4 directing expression of the sponge (Gal4 > 31a sponge). (A) repo‐Gal4, (B) Syb‐Gal4, (C) elav‐Gal4, (D) Insc‐Gal4, Df(3L)H99 indicate flies carrying one copy of this deletion, to limit apoptosis in the presence of the UAS‐miR‐31a sponge or UAS‐GFP.
  • E, F
    Number of anti‐repo‐positive glia following adult‐specific depletion of miR‐31a. Flies carrying Gal80 ts with Insc‐Gal4 (E) or miR‐31a‐Gal4 (F) and the UAS‐miR‐31a sponge or UAS‐GFP were raised at 18°C until adults had eclosed, and were then shifted to 29°C to allow Gal4 activity. UAS‐GFP was used as a control. Flies were examined 7 days after Gal4 activation. Data were analysed using an unpaired two‐tailed Student's t‐test. Error bars represent SEM.
  • G, H
    Number of anti‐repo‐positive glia in brains at 7 days. UAS‐GFP was used as a control for expression of UAS‐miR‐31a transgene. Ctrl: Canton S control. 31a KO/KO indicates the homozygous mutant. Data were analysed using one‐way ANOVA with post hoc Tukey analysis. Error bars represent SEM. (G) Insc‐Gal4 was used to direct transgene expression. (H) The miR‐31a‐Gal4 allele was used to direct transgene expression. Gal4/+ indicates miR‐31a‐Gal4 allele in trans to wild‐type. miR‐31a‐Gal4/KO indicates the Gal4 allele in trans to the deletion allele.