Skip to main content
. 2017 Mar 20;36(9):1215–1226. doi: 10.15252/embj.201695861

Figure EV2. Rchy1, the target of miR‐31a, causes death of glia by apoptosis (related to Figs 2 and 3).

Figure EV2

  1. Left panel: number of repo‐expressing and lineage‐tagged GFP+ cells. Right panel: lineage‐tagged GFP+ cells with active repo‐Gal4. Data are presented as a ratio of the total number of GFP cells in the central brain. Error bars represent SEM.
  2. Number of glia in miR‐31a mutants at 7 days with and without Df(3L)H99. Introducing Df(3L)H99 prevented glial loss. Data were analysed with an unpaired Student's t‐test. Error bars represent SEM.
  3. Alignment of fly and human Rchy1 proteins showing regions of sequence similarity. The antibody to human Rchy1 was raised against a peptide containing residues 87–167.
  4. The number of Rchy1‐expressing cells in the central brain was greater in the miR‐31a mutants than in Canton S controls (88 ± 0.4 vs. 17 ± 3). Data were analysed with an unpaired Student's t‐test. Error bars represent SEM.
  5. Activated caspase‐3‐positive (green) glial cells stained with anti‐repo (purple) are observed in the brains of 2‐days post‐eclosion adult repo‐Gal4 > UAS‐CG16947 brains. White arrowheads point to anti‐repo‐positive cells that are activated caspase‐3‐positive.