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. 2017 Mar 20;36(9):1215–1226. doi: 10.15252/embj.201695861

Figure 3. An E3 ubiquitin ligase gene, CG16947, is the target of miR‐31a .

Figure 3

  • A–F
    Number of anti‐repo‐positive glia in adult brains at 7 days. Glia counts in the experimental samples are represented as a percentage of the average number of glia in central brains of the indicated controls. Data were analysed using one‐way ANOVA with post hoc Tukey analysis (A, C, D, F) or with an unpaired Student's t‐test (B, E). Error bars represent SEM. (A) The UAS‐CG16947 RNAi transgene without a Gal4 driver was used as a control. miR‐31a KO/KO indicates the homozygous deletion mutant. A UAS‐GFP transgene was used as a control for expression of the RNAi transgene with Insc‐Gal4 in the mutant background. ns: not significant. See also Appendix Fig S1 and Table EV1. (B) Expression of UAS‐GFP or UAS‐CG16947 RNAi using Insc‐Gal4 cells in an otherwise normal background. (C) The UAS‐CG16947 transgene without a Gal4 driver was used as a control. UAS‐GFP was used as a control for expression of the UAS‐CG16947 transgene with Insc‐Gal4 in an otherwise normal background. (D) All samples carried one copy of the miR‐31a‐Gal4 allele. KO;GFP indicates the deletion allele and a UAS‐GFP transgene. KO,CG16947 RNAi indicates the deletion allele and the UAS‐RNAi transgene to deplete CG16947 mRNA. (E) All flies carried the miR‐31a‐Gal4 allele. KO indicates the deletion allele. (F) The UAS‐CG16947 transgene without a Gal4 driver was used as a control. UAS‐GFP in the mutant background was used for comparison to expression of UAS‐CG16947 transgene with miR‐31a‐Gal4 in an otherwise normal background.
  • G, H
    Optical sections of 7‐days adult brains labelled with antibody to Rchy1 protein. miR‐31a‐Gal4 was used to drive the expression of UAS‐Histone‐RFP. Glia were visualized with anti‐repo. More cells expressed Rchy1, Histone‐RFP and repo in the mutant brains (miR‐31a‐Gal4/KO > RFP) than in the heterozygous controls (miR‐31a‐Gal4/+ > RFP, white arrowheads). (H) Higher magnification images from the samples in (G).
  • I
    Number of anti‐repo‐positive glia in 7‐days post‐eclosion brains from controls expressing UAS‐GFP or UAS‐CG16947 in glia under repo‐Gal4 control. Data were analysed using an unpaired Student's t‐test. Error bars represent SEM.