Figure EV4. Active division of progenitor cells is observed in the adult brain of Drosophila melanogaster (related to Fig 5).

1. EdU‐labelling experiment. Overview confocal stack images of EdU and repo immunoreactivity in the central brains of control and miR‐31a mutant brains (mutant).
2. Raw counts of anti‐repo‐positive glia in the central brain in glial ablation experiments where flies carrying repo‐Gal4 and Gal80 ^ts were reared at 18°C until eclosion to keep Gal4 inactive. This was followed by 2 days at 29°C to express UAS‐hid or UAS‐GFP as a control. Left panels: flies were examined immediately after 2 days of transgene expression. Right panels: flies were allowed to recover for 14 days at 18°C before processing. Data were analysed using an unpaired t‐test (two‐tailed). ns: not significant. Error bars represent SEM.