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. 2017 Mar 24;18(5):712–725. doi: 10.15252/embr.201643585

Figure EV1. GPR37 is required for Wnt/β‐catenin signaling.

Figure EV1

  • A, B
    qPCR representing the knockdown efficiencies of the indicated single siRNAs or siRNA pool (siGPR37) used in Fig 1.
  • C–F
    Reporter assays for Wnt (C), TGFβ (D), BMP4 (E), or FGF (F) signaling in HEK293T cells upon knockdown of GPR37 or LRP5/6. Cells were transfected as described in the Materials and Methods section, and stimulated with conditioned media (C) or the indicated recombinant proteins (D–F) (mean values ± SD, n = 3).
  • G
    Topflash reporter assay in HEK293T cells transfected with either control vector or GPR37. Cells were treated for 24 h with recombinant prosaptide (0–3 μM) in the presence or absence of Wnt3a‐conditioned media (mean values ± SD, n = 3).