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. 2017 Mar 22;18(5):765–780. doi: 10.15252/embr.201643146

Figure EV2. Atg4 association with the PAS does not require components of the Atg1 complex, Atg9 cycling system and PI3K complex.

Figure EV2

  1. Fluorescence microscopy images showing the subcellular localization of Atg4‐GFP in atg13∆ (SAY030), atg2∆ (SAY109), atg9∆ (SAY016), atg18∆ (SAY017), atg6∆ (SAY013), and atg14∆ (SAY110) strains analyzed as in Fig 1. White arrows highlight Atg4‐GFP puncta. DIC, differential interference contrast. Scale bars, 5 μm.
  2. Percentage of cells, in the experiments shown in panel (A), that display an Atg4‐GFP punctate structure. Data represent the average of three independent experiments ± SD.
  3. Subcellular localization of Atg4‐GFP in double knockout cells lacking Atg1 and components of the conjugation systems leading to the formation of Atg8‐PE: atg7∆ (SAY032), atg3∆ (SAY031), atg8∆ (SAY033), atg10∆ (SAY035), atg12∆ (SAY134), and atg16∆ (SAY135). Cells were grown and imaged as in Fig 1. DIC, differential interference contrast. Scale bars, 5 μm.