The SAY130 strain (Pho8∆60
pho13∆ atg4∆) carrying plasmids expressing Atg4, Atg4
PD, Atg4
APEAR, Atg4
cLIR, and Atg4
APEAR,cLIR was analyzed as in Fig
2E. Data represent the average of three independent experiments ± SD. Significant differences (
P < 0.05) between cells expressing WT Atg4 were calculated using the paired two‐tailed Student's
t‐test, and they are indicated with the # symbol.