Figure 3. Depletion of API5 sensitizes HeLa cells to PFT, but not to other inducers of apoptosis.

• A, B
  ShControl and shAPI5 cells were treated for 24 h with α‐toxin (150 ng/ml), staurosporine (STS, 125 nM), TNF‐α (20 ng/ml)/CHX, camptothecin (CPT, 4 μM), etoposide (ETO, 50 μM), cisplatin (Cis, 40 μM) or brefeldin A (BrefA, 5 μM). Cells were harvested and labelled with propidium iodide for cell death assay (B) or lysed for Western blot analysis (A) (* indicates processed caspase‐2 and PARP). The Ponceau staining of the entire membrane is shown below. For (B) n = 3, two‐way ANOVA with a Bonferroni test, ***P‐value < 0.001.

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